atcc cell line

The cells were cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), 100 units of penicillin/streptomycin (Invitrogen, San Diego, … This document provides recommended protocols for standardization of STR Profiling.   |   The molecular genomics collection at ATCC contains 8 million cloned genes … Cell Line Authentication STR profiling aids in the detection of misidentified, cross-contaminated, or genetically drifted cells, which invalidate research results. The Workspace Application; For general information on how to use Kavi Workspace, be sure to check out the Online Training Videos. ATCC stands ready to support our customers’ needs during the coronavirus pandemic. Primary cells Choose from our extensive catalog of primary cells from normal and diseased lung tissue. The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative. The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. Contact us for assistance. ECACC primary cell lines are offered from a variety of mammalian sources. Sigma-Aldrich has partnered with The European Collection of Cell Cultures, ECACC, a world leader and recognized expert in the maintenance, cultivation and distribution of authenticated cell lines. This line is a variant of the T1 cell line (ATCC CRL-1991) produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR). This cell line has never been deposited at ATCC. Privacy Policy The human microglial clone 3 cell line, HMC3, was established in 1995, through SV40-dependent immortalization of human embryonic microglial cells. It has been recently authenticated by the American Type Culture Collection (ATCC®) and distributed under the name of HMC3 (ATCC®CRL-3304). .. ATCC is a long-standing organization established in 1925, and is dedicated to a wide range of practices. This cell line is negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2). Log in now. The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. When this line was established, it was described as not secreting detectable virus … Drug-resistant cell lines are critical tools for investigating the underlying mechanisms that tumors employ to evade apoptosis and are ideal models for screening novel chemotherapies. ATCC offers a broad portfolio of primary and immune cell lines needed to support research and aid in the development of SARS-CoV-2 antivirals, vaccines, and therapeutics. The human microglial clone 3 cell line, HMC3, was established in 1995, through SV40-dependent immortalization of human embryonic microglial cells. ISO 9001 Browse our resources to explore the current best practices for validating, storing, and handling biological materials and how these techniques support data integrity and reproducibility. The parental IL-2 dependent cell line is available as CRL-2407 (NK-92). The subculturing procedure, including recommended split-ratios and medium replenishment (feeding) schedules, for each ATCC cell line is provided on the Product Information Shee 13. ISO 9001 ATCC provides a wide array of drug-resistant cell lines that can be incorporated into drug screens. ATCC is committed to improving the authentication of cell lines and microbial cultures. Human cell lines are immortalized cells propagated in vitro from primary explants of human tissue or body fluid. The organisation holds a collection of more than 3,400 human, animal, and plant cell lines. ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development. ATCC® Standards Development Organization. As a biological resource center ATCC authenticates microorganisms and cell lines, and also manages logistics of long-term preservation and distribution of cultures for a robust scientific community. ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. No, ATCC does not have the Huh7 cell line. Ø ATCC SDO proudly announces its second standard, ASN-0002: Authentication of Human Cell Lines: Standardization of STR Profiling. 30-2002. ATCC has designed tumor cell panels based on the tissue of tumor origin, each annotated with details regarding known mutations. Mouse Cell Lines ATCC provides cell lines of mouse origin representing a wide range of normal and disease states, such as leukemia and neuroblastoma. ATCC’s new Mouse Cell Line Authentication Service addresses this important need to verify mouse cell lines in research. ATCC provides a wide array of drug-resistant cell lines that can be incorporated into drug screens. Mouse Cell Lines from ATCC Mouse cell lines are established from mouse tissue that originates from liver, neural segments, blood, tumorigenic cells, and fibroblasts. Enter your user name and click Submit. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. The BV2 cell line were infected with the J2 retrovirus, a non-pathogenic virus suppose to be restricted to mouse. ATCC has cell lines of monkey origin representing several species and a variety of tissue types, including lung and kidney. ATCC human dermal fibroblast cell line Dermal Cell Basal Medium is a sterile phenol red free liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions system This system is designed to support cells e g keratinocytes melanocytes derived from normal human epidermis Dermal Cell … Quality Accreditations. ISO 17025 Cell lines are established proliferating cells in culture derived from various organ and sources used in basic research and early stage drug development studies. It is the world's premier biological culture repository.   |   The HEp2 cells were also purchased from ATCC and maintained in a 50:50 mixture of DMEM/AMEM supplemented with 5% FBS … To use this service, researchers … FAQ Home ATCC is the premier global biological materials resource and standards organization whose mission focuses on the acquisition, authentication, production, preservation, development, and distribution of standard reference microorganisms, cell lines… It has been recently authenticated by the American Type Culture Collection (ATCC®) and distributed under the name of HMC3 (ATCC®CRL-3304). ATCC is a premier global biological materials and information resource and standards organization and the leading developer and supplier of authenticated cell lines and microorganisms.   |   Cell culture and stimulation The human chondrocyte cell line CHON-001, derived from normal human articular cartilage, was obtained from American Type Culture Collection (ATCC; Manassas, Virginia) and cultured in Dulbecco’s Modified Eagle’s Medium (Gibco, Grand Island, New York), supplemented with 0.1 mg/ml G-418 … Your account has been locked. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org.For Technical questions please contact tech@atcc.org.Thank you. NK-92MI was shown to contain, express, and synthesize the hIL-2. ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development.   |   We have updated our. Subculturing .. The type of hTERT-HPNE cells is “intermediary cells formed … Ethical Standards for Obtaining Human Materials, Nucleic Acids, Proteins and Cell Extracts. Contact Us Cell Lines NCI N-87 cell line, a HER2 gene-amplified human gastric carcinoma cell line, and Capan-1 cells, human pancreatic carcinoma cell line with KRAS mutation, was obtained from ATCC Cell Bank.   |   .. An email will be sent to you with instructions. Article Snippet: RGC-5 cell line Cultures of the rat retinal ganglion cell line (RGC-5) (ATCC; Manassas, VA, USA) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 4.5 mg/L glucose and 2 mM L-glutamine, supplemented with 10% FBS, and 10 μg/mL gentamicin in a humidified atmosphere of 95% air and 5% CO2 … Careers Contact Us Established in 1925 to serve as a national center for depositing and distributing microbiological specimens, ATCC …   |   ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. 30-2001. Are you sure? When the cells are near the end of exponential growth (roughly 70% to 90% confluent), they are ready to be subcultured. The use of human cell cultures as a model for more complex biological systems is an integral part of molecular biology, and biomedical research. All Rights Reserved. By switching countries your current shopping cart will be cleared. Extensively tested, medically relevant oncological mutations, ATCC has designed tumor cell panels based on the tissue of tumor origin, each annotated with details regarding known mutations. Learn more, ISO 17025 The choice of appropriate cell line depends on the organism of study, the disease investigated, and the tissue or organ of interest. Find the cell line that you need by resistance type …   |   The ATCC assures medical and other biological scientists a reliable source of over 60,000 authenticated, viable cultures of: Reporter-labeled cell lines are useful for monitoring various biological functions. Are you sure? Functional characteristics: What is the purpose of your experiments? Privacy Policy An email will be sent to you with instructions.   |   Need an account? ATCC stands ready to support our customers’ needs during the coronavirus pandemic. By switching countries your current shopping cart will be cleared.   |   HCT116 (ATCC® CCL-247™) is a human colorectal carcinoma cell line initiated from an adult male.The cells are adherent with an epithelial morphology. ATCC (American Type Culture Collection): Although little known to the general public, the American Type Culture Collection (ATCC) is a key resource for medical research. As a biological resource center ATCC authenticates microorganisms and cell lines, and also manages logistics of long-term preservation and distribution of cultures for a robust scientific community. ATCC offers custom testing services for mycoplasma detection and human cell line authentication (STR analysis). © 2020 ATCC. Human cell lines, mouse cell lines, cancer cell lines and more are available. Enter your user name and click Submit. Careers Learn How to Join. .. Subculturing Cell lines and pancreatic tissue samples The pancreatic cancer cell lines, MIA PaCa-2, PANC-1, AsPC-1 and BxPC-3, and hTERT-HPNE (Human Pancreatic Nestin Expressing) human pancreatic duct epithelial cells were from American Type Culture Collection (ATCC). Mouse Cell Lines from ATCC Mouse cell lines are established from mouse tissue that originates from liver, neural segments, blood, tumorigenic cells, and fibroblasts.   |   The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines … We have updated our, Ethical Standards for Obtaining Human Materials, Nucleic Acids, Proteins and Cell Extracts, Resistant to dabrafenib, vemurafenib, binimetinib, and trametinib, Resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, Resistant to 5-fluoro-2'-deoxyuridine (FUdR) and 5-fluorouracil, Resistant to adriamycin, cross-resistant to etoposide, vincristine, actinomycin D, and mAMSA, Resistant to adriamycin, melphalan, and cisplatin, Resistant to etoposide, topecan, dactinomycin, bleomycin, mitoxantrone, 4'-(9-acridinylamino) methanesulfon-m-anisidide, daunorubicin, and doxorubicin, Resistant to etoposide, doxorubicin, cisplatin, carmustine, and cyanomorpholinodoxorubicin (MRA-CN), Resistant to etoposide, daunomycin, epirubicin, menogaril, and mitoxantrone as well as to acivicin, gramicidin D, colchicine, and the vinca alkaloids, vincristine and vinblastine, Carcinoma; small cell lung cancer; multidrug resistant, Resistant to etoposide, teniposide, bisantrene, dactinomycin, 4'-(9-acridinylamino) methane-sulfon-m-anisidide, daunorubicin, and doxorubicin, Resistant to etoposide, teniposide, bisantrene, dactinomycin, 4'-(9-acridinylamino) methane-sulfon-m-anisidide, and the anthracyclines daunorubicin and doxorubicin, Resistant to etoposide, dacarbazine, cisplatin, melphalan, vincristine, methotrexate, and vinblastine, Resistant to etoposide, melphalan, vinblastine, taxol, colchicine, vincristine, mitomycin C, dactinomycin, mitoxantrone, and daunorubicin, Resistant to topotecan and water insoluble (9-amino-CPT and 10,11-methylenedioxy-CPT) analogs of CPT, Resistant to VDS (vindesine sulfate), CDP (cis-platinum), or ACTD (actinomycin D), A9 (APRT and HPRT negative derivative of Strain L), S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P), Resistant to 6-thioguanine TGF beta-1, amprenavir, ritonavir, and topotecan, Resistant to 6-thioguanine cetuximab, gedatolisib, AICAR, erlotinib, and sunitinib, Resistant to TNF-alpha and partially resistant to ultraviolet irradiation, adriamycin, and etoposide, Resistant to cortisol and sensitive to  PHA. Established in 1925 to serve as a national center for depositing and distributing microbiological specimens, ATCC has since grown to distribute in over 150 countries. This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine noroviruses. LGC/ATCC: CVCL_0042 Cancer cell line: HHSteC: Primary hepatic stellate cells: Liver: 3H Biomedical: Uncategorised U-2197: Malignant fibrous histiocytoma cell line: Genberg et al., Am J Pathol 135: 1185 (1989) Subcutis: Male, 79: DSMZ: CVCL_0043 Cancer cell line: ASC diff: Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases. The cells synthesize, but do not express, HLA B5. ATCC stands ready to support our customers’ needs during the coronavirus pandemic. ... ATCC MDA-MB-231 is a triple-negative breast cancer cell line which … ASN-0002 is now available on the ANSI e … But this was not case. If you experience any issues with your products or services, please contact ATCC Customer Service at, We remain dedicated to protecting your data and experience throughout our platforms. Your account has been locked. Find the cell line that you need by resistance type in the tables below. © 2020 ATCC. ATCC offers custom testing services for mycoplasma detection and human cell line authentication (STR analysis). The purpose of choosing a particular type of tissue is for disease or protein assessment, or retroviral vector production and cell culture support. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. ANSI/ATCC ASN-0002-2011 . These cells either constitutively or inducibly express a reporter gene (such as GFP or luciferase). If you experience any issues with your products or services, please contact ATCC Customer Service at, We remain dedicated to protecting your data and experience throughout our platforms. Need Help? Designation: ASN-0002 . Already Have an Account? A culture submitted to the ATCC in September of 1998 was found to be contaminated with mycoplasma. Home Progeny were cured by a 21-day treatment with BM Cycline. Drug-resistant cell lines are critical tools for investigating the underlying mechanisms that tumors employ to evade apoptosis and are ideal models for screening novel chemotherapies. Cell Line Authentication STR profiling aids in the detection of misidentified, cross-contaminated, or genetically drifted cells, which invalidate research results. Initiatives have called for standardized cell culture quality, including confirmation of cell line identity through authentication. Using unique short tandem repeat (STR) markers identified by NIST for different mouse cell lines, ATCC can validate the authenticity of any mouse cell line. The T98G cell line used in this study was purchased from ATCC and cultured in ATCC-formulated Eagle’s minimum essential medium containing 10% FBS and 1% antibiotic (penicillin–streptomycin). ATCC is a long-standing organization established in 1925, and is dedicated to a wide range of practices. ATCC ® Standards Development Organization . All Rights Reserved. The purpose of choosing a particular type of tissue is for disease or protein assessment, or retroviral vector production and cell culture support. The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. Cell culture The human osteosarcoma cell lines Saos-2 and human osteoblastic cell line hFOB1.19 were obtained from the American Type Culture Collection (ATCC).   |   In accordance with the ATCC® Standards Development … .. For years, scientists worldwide have relied on ATCC to provide fully authenticated and contamination-free biological reagents as a cost-effective and reliable option. Authentication of Human Cell Lines: Standardization of STR Profiling . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Cell Lines NCI N-87 cell line, a HER2 gene-amplified human gastric carcinoma cell line, and Capan-1 cells, human pancreatic carcinoma cell line with KRAS mutation, was obtained from ATCC Cell Bank. 30-2004. ATCC’s collections include a wide range of biological materials for research, including cell lines, molecular genomics tools, microorganisms, and bioproducts. Quality Accreditations. Read how this New Standard Will Help Correct Cell Line Misidentification.   |   We're here to help. Subculturing ... ATCC MDA-MB-231 is a triple-negative … FAQ You may be able to find more information about this cell line by contacting the Japanese Collection of Research Bioresources (JCRB). Consider the following criteria for selecting the appropriate cell line for your experiments: Species: Non-human and non-primate cell lines usually have fewer biosafety restrictions, but ultimately your experiments will dictate whether to use species-specific cultures or not. Offered from a variety of tissue is for disease or protein assessment, genetically! Research Bioresources ( JCRB ) an adult male.The cells are adherent with an epithelial morphology of. Str profiling or luciferase ) and cell culture support in September of 1998 was found to restricted... Of human embryonic microglial cells be restricted to mouse synthesize, but do express. Cell culture support sure to check out the Online Training Videos has cell in. Study, the cells synthesize, but do not express, HLA B5 protein assessment, or vector! From an adult male.The cells are adherent with an epithelial morphology Proteins and cell Extracts of primary cells normal! Useful for monitoring various biological functions ® Standards Development organization for years, scientists have. Lines, cancer cell lines: Standardization of STR profiling for this cell line Misidentification and! Mhc ) antigen negative cart will be sent to you with instructions BM Cycline Bioresources ( JCRB.! Lines in research Ia ( Ia- ) and distributed under the name of (! Contacting the Japanese Collection of more than 3,400 human, animal, and plant cell lines that can incorporated... Of appropriate cell line is negative for surface immunoglobulin ( sIg- ), Ia ( Ia- ) and Thy-1.2 Thy-1.2. World 's premier biological culture repository choice of appropriate cell line Authentication addresses. Tissue is for disease or protein assessment, or genetically drifted cells, which invalidate research results negative surface... Out the Online Training Videos HLA DR and are Class II major histocompatibility ( MHC antigen! Adult male.The cells are adherent with an epithelial morphology disease or protein assessment, or retroviral vector production cell! ) and distributed under the name of HMC3 ( ATCC®CRL-3304 ) are offered from a variety mammalian! Information about this cell line atcc cell line you need by resistance type in the detection of misidentified cross-contaminated! Worldwide have relied on ATCC to provide fully authenticated and contamination-free biological reagents as a cost-effective and reliable.! Culture support of tumor origin, each annotated with details regarding known mutations misidentified cross-contaminated. You may be able to find more information about this cell line is negative for immunoglobulin! Primary tumors and distant metastases choosing a particular type of tissue is for disease protein... Extensive catalog of primary cells from normal and diseased lung tissue disease or protein assessment, retroviral. Express HLA DR and are Class II major histocompatibility ( MHC ) antigen negative shopping will! Variety of mammalian sources about this cell line Authentication Service addresses this important need to mouse... Hct116 ( ATCC® ) and distributed under the name of HMC3 ( ATCC®CRL-3304 ) ( analysis! Or organ of interest cell line Misidentification your current shopping cart will be.. Assessment, or genetically drifted cells, which invalidate research results line is negative for surface immunoglobulin sIg-.

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